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Is there horse in my wors?
Or donkey, goat or kangaroo…

Did you know FACTS can detect meat adulteration?
February 2013

How sure are you that meat adulteration, i.e., any species that may be in your product which you may not be aware of has not affected your supply chain? DNA screening for a range of meat species can eliminate doubt.

It is no secret that the recent meat adulteration scandal has shaken the food industry over the last few weeks, presenting a plethora of new challenges to food manufacturers and compromising consumer confidence in the functioning of the meat supply chain, both locally and globally. The shocking issue, which came to light on 15 January 2013, involved foods labelled as beef in the EU, which was found to contain undeclared horse meat, as well as other unlisted meats such as pork. Consequently, a number of big food industry names have been caught off guard. These include the UK supermarket chains Tesco, Iceland and Lidl, the German discounter Aldi, the frozen-foods processor Findus and the global fast food giant Burger King, all of which have all been implicated in marketing fraudulent meat.

Earlier this month, the EU commission approved plans to carry out random DNA testing on meat products throughout member states and 11 of the UK's largest food suppliers have declared that they are "working around the clock to complete the most comprehensive testing of processed beef products ever undertaken, anywhere in the world.”

What may have been largely overlooked in this case is that such meat adulteration scandals are not limited to horsemeat, and they are certainly not limited to the EU.

A recent meat authentication study conducted by FACTS food scientist, Dr. Donna Cawthorn, hit headline news in December 2012, when it was revealed that 68% of 139 processed meat products obtained from the South African market contained species not declared on the label, including undeclared pork, donkey, goat and water buffalo (see related publication). In a related story, the problem of species substitutions involving game meat served at local restaurants was simultaneously exposed.

Butchers mislabel meats.Daily News, 10 December 2012.
The meat you eat is not always what it seems. Cape Times, 10 December 2012.
Pigging out on Warthog. Cape Times, 10 December 2012.
One man's meat, another's poison. Independent Online, 12 December 2012.
Wat's in boerie op jou braai?Beeld, 13 December 2012.
Etiket sÍ nie wat is regtig in braaiwors. Die Burger, 14 December 2012.
Is there donkey in that wors? The Witness, 15 December 2012.

Ensuring meat authenticity

While knowing your meat suppliers and auditing them regularly may be an obvious step in protecting the authenticity of meat products, in many cases food fraud cannot be discovered by following a paper trail and detection requires ‘state of the art’ scientific analysis. Today, DNA-based techniques are considered the most appropriate methods for making species identifications as DNA is present and is identical in all tissue types, is relatively stable even at high temperatures and because the diversity afforded by the genetic code allows the differentiation of very closely-related species.

Over the past few years, FACTS has developed a large number of DNA-based methods for the identification of meat species, both in single ingredient commodities (using DNA sequencing) and in complex food matrices (using species-specific detection methods) (see our full list of services below). The detection of the compete substitution of a single meat species with another is one of the more simple scenarios for identifying food fraud. In general, DNA sequencing conducted on a queried sample will produce a DNA sequence or ‘fingerprint’ which can compared to a set of known reference sequences in a credible genetic database. The identity of the specimen can thereby be established, which is either the same or different to the one expected. Investigating the partial substitution or adulteration of meat is considerably more difficult, since it is normally necessary to know the possible identity of the adulterant before it can be detected. To help alleviate the aforementioned difficulties, FACTS is very excited to have recently optimised an animal species screening method which allows the detection of up to 24 different animal species in a single reaction (more details below). This method, which relies on the use of species-specific DNA probes to detect certain target DNA sequences, will significantly reduce the costs and labour required to ensure product authenticity.

Food fraud is indeed a food industry issue. Consumers are highly reliant on the accurate and complete declaration of food constituents to enable them to make product choices that are consistent with their lifestyles. Brand loyalty can be severely compromised should this be found not to be the case. As such, DNA testing can be of great value for the routine monitoring of meat product authenticity and should be conducted whenever a case of meat adulteration or contamination is suspected.

ANIMAL SPECIES DETECTION AND IDENTIFICATION SERVICES

1. Screening for animal species (screening for multiple animal species in a single reaction)

DNA array method for the qualitative detection and identification of multiple animal species based on the analysis of conserved regions of mitochondrial DNA:

14-species DNA LCD-array
Qualitative detection of the following species:
  24-species DNA LCD-array
Qualitative detection of the following species:
Beef Bos spp.   Beef Bos spp.
Water buffalo Bubalus bubalis   Water buffalo Bubalus bubalis
Pork Sus scrofa   Pork Sus scrofa
Sheep Ovis aries   Sheep Ovis aries
Goat Capra hircus   Goat Capra hircus
Horse Equus caballus   Horse Equus caballus
Donkey Equus asinus   Donkey Equus asinus
Hare Lepus europaeus   Hare Lepus europaeus
Rabbit Oryctolagus cuniculus   Rabbit Oryctolagus cuniculus
Chicken Gallus gallus   Chicken Gallus gallus
Turkey Meleagris gallopavo   Turkey Meleagris gallopavo
Goose Anser albifrons   Goose Anser albifrons
Mallard Duck Anas platyrhyncos   Mallard Duck Anas platyrhyncos
Muscovy Duck Cairina moschata   Muscovy Duck Cairina moschata
      Pheasant Phasianus cholchicus
      Ostrich Struthio camelus
      Bison Bos bison
      Kangaroo Macropus giganteus / M. rufus
      Springbok Antidorcas marsupialis
      Fallow Deer Dama dama
      Red Deer Cervus elaphus
      Axis Deer Cervus axis / Axis axis
      Reindeer Rangifer tarandus
      Roe Deer Capreolus capreolus

2. DNA sequencing for species identification – identification in single ingredient commodities

DNA sequencing of PCR products can be performed and compared to known DNA sequences as a means of confirming the identification of the following species:
1.1. Fish species identification
1.2. Crustacean species identification
1.3. Mollusc species identification
1.4. Bovine (beef) species identification
1.5. Porcine (pork) species identification
1.6. Ovine (sheep) species identification
1.7. Game species identification (e.g. kudu, impala, springbok, blesbok, bontebok, duiker etc.)
1.8. Goat species identification
1.9. Chicken species identification
1.10. Turkey species identification
1.11. Ostrich species identification
1.12. Duck species identification
1.13. Crocodile species identification
1.14. Donkey species identification
1.15. Horse species identification
1.16. Buffalo species identification (African buffalo Syncerus Caffer and water buffalo (Bubalus bubalis)
1.17. Rabbit species identification

3. Species-specific detection - detection in mixed ingredient commodities (detection of adulteration)

2.1. Pork-specific detection - for the detection and identification of Sus spp.
2.2. Chicken-specific detection - for the detection and identification of Gallus gallus (chicken)
2.3. Turkey-specific detection – for detection and identification of Meleagris meleagridis (turkey)
2.4. Bovine-specific detection – for the detection of Bos Taurus (beef)
2.5. Ovine-specific detection – for the detection and identification of Ovis aeries (sheep)
2.6. Goat-specific detection – for the detection and identification of Capra hircus (goat)
2.7. Ostrich-specific detection – for the detection of Struthio camelus (ostrich)
2.8. Fish-specific detection – for the detection of teleosts (bony fish)-species
2.9. Crustacean-specific detection – for the detection of crustacean species

Please feel free to contact us should you have any queries regarding the animal species authentication and species detection services offered.